DNA+Extraction



= DNA Extraction = Add 20 ml of detergent to 20 g non-iodized salt and 180 ml of distilled water. Add 5 g of meat tenderizer to 95 ml of distilled water. Add 5 ml of distilled water to 95 ml of ethanol (alcohol). Rubbing alcohol can be substituted. Place this solution on ice, the colder the better.
 * Detergent/Salt Solution **
 * //The detergent salt solution is used to break down the fat and proteins that make up the cell membrane. The salt causes the phosphate ends of the DNA to come closer together which will make it easier to precipitate out of solution.// ||
 * Meat Tenderizer Solution – (Not used in this Procedure) **
 * //Enzymes in the meat tenderizer will break down proteins// ||
 * Ethanol Solution **
 * //Alcohol is used to precipitate the DNA. Because DNA is soluble in water, alcohol (ethanol) causes the DNA to precipitate and come out of the solution. DNA will rise into the alcohol layer.// ||

1. Add 2 tsp of untoasted wheat germ to 150 ml beaker. 2. Measure 50 ml hot water (45C) in graduated cylinder and add to beaker of wheat germ. 3. Stir briskly for 2 minutes with glass stirring rod. 4. Measure 5 ml of soap/salt solution in a graduated cylinder and add to wheat germ beaker. Stir GENTLY for 1 minute. (avoid bubbles or foam) 5. Add 5 ml MORE soap/salt mixture to wheat germ and stir GENTLY for 1 minute. 6. Pour into a test tube ~1/3 full. 7. Pour cold ethanol slowly down the side of the test tube to form ~1.5 cm layer on top. (teacher may do this step for students) 8. Let the mixture sit for 1 minute. 9. Use a stirring rod or wooden stick to spool and draw the DNA into the alcohol. Can remove DNA and put on paper towel.
 * Procedure **